Bioinformatics Analysis and Expression Profiling of the Aluminum Transporter HmALMT11 in Hydrangea macrophylla
;Aluminum-activated malate transporter(ALMT)gene family,as a widely distributed gene family in plants,the encoded protein plays an important role in regulating plant root acid secretion and response to aluminum ions.In order to clarify the sequence characteristics and expression features of H.macrophylla Endless Summer(HmALMT11)under abiotic stress,further exploring the biological functions of H.macrophylla Endless Summer,and providing a theoretical basis for the subsequent identification of the function of HmALMT11.HmALMT11 gene was cloned from the leaves of Hydrangea macrophylla Endless Summer,and the bioinformatics,subcellular localiza-tion and aluminum stress response analysis of this gene were also carried out.HmALMT11 contained an open read-ing frame of 1 590 bp and encoded 529 amino acids,with a molecular weight of 59.1 ku and a theoretical isoelectric point of 9.10 was an unstable basic protein containing 6 transmembrane domains.The subcellular localization analysis revealed that the protein encoded by HmALMT11 was specifically targeted to the plasma membrane.The qRT-PCR a-nalysis revealed that treatments with both low(100 μmol/L)and high(800 μmol/L)concentrations of Al2(SO4)3 increased the expression level of HmALMT11 in the roots,stems,and leaves of Hydrangea macrophylla Endless Sum-mer.Notably,the highest expression level was observed in the roots,demonstrating a clear tissue-specific expression pattern.These results suggested that HmALMT11 protein might play an important role during the regulation of alu-minum tolerance in hydrangea.
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