两种花色绿绒蒿MiUGT79B1和MpUGT79B1基因的克隆及表达分析
Cloning and Expression Analysis of MiUGT79B1 and MpUGT79B1 Genes from Two Varieties of Meconopsis
周麟 1李拓键 1屈燕1
作者信息
- 1. 西南林业大学园林园艺学院,云南昆明 650224
- 折叠
摘要
为探究尿苷二磷酸糖基转移酶(UGT)在不同花色绿绒蒿中的序列特征及其与绿绒蒿花色的关系,以黄色花的全缘叶绿绒蒿和红色花的红花绿绒蒿为研究材料,从2种花色绿绒蒿的全长转录组数据中各筛选1个UGT基因,根据转录组KEGG注释结果,将这2个基因分别命名为MiUGT79B1和MpUGT79B1,并对其进行克隆、生物信息学及RT-qPCR分析.结果表明:MiUGT79B1和MpUGT79B1基因ORF长度为1 314,编码437个氨基酸.MiUGT79B1和MpUGT79B1蛋白的结构均主要由α-螺旋和无规则卷曲构成,属于亲水性稳定蛋白.系统进化树和多序列比对分析发现,MiUGT79B1和MpUGT79B1与鸦片罂粟、火罂粟、野罂粟和博落回的亲缘关系最近.RT-qPCR分析显示,MiUGT79B1和MpUGT79B1均在花蕾期高表达,MiUGT79B1表达趋势为先降低后升高,MpUGT79B1表达趋势为先降低后保持平缓.2种花色绿绒蒿UGT基因表达水平与总黄酮累积模式均呈显著正相关.结果为进一步深入研究MiUGT79B1和MpUGT79B1基因在绿绒蒿花瓣呈色中的功能及其花色分子育种提供理论基础.
Abstract
To investigate the sequence characteristics of uridine diphosphate glycosyltransferase in different flower color of Meconopsis and its relationship with the flower color of Meconopsis.The yellow-flowered Meconopsis integrifolia and the red-flowered M.punicea were used as study materials,and one UGT gene was screened from the full-length transcriptome data of each of the two flower colors of Meconopsis.Based on the results of KEGG an-notations of the transcriptomes,these two genes were named MiUGT79B1 and MpUGT79B1,and they were cloned,and analyzed by bioinformatic and RT-qPCR.The results showed that the ORF length of MiUGT79B1 and MpUGT79B1 genes was 1 314,encoding 437 amino acids.Both MiUGT79B1 and MpUGT79B1 proteins were hy-drophilic stabilizing proteins with their structures consisting mainly of α-helices and random coil.Phylogenetic tree and multiple sequence comparison analyses revealed that MiUGT79B1 and MpUGT79B1 were most closely related to Papaver somniferum,P.californicum,P.nudicaule,and Macleaya cordata.RT-qPCR analysis showed that Mi-U GT79 B1 and MpUGT79B1 were highly expressed in the bud stage,MiUGT79B1 expression tended to decrease first and then increase,and MpUGT79B1 expression tended to decrease first and then remain stable.There was a significant positive correlation between the expression level of UGT gene and the accumulation pattern of total fla-vonoids.The results of this study provide a theoretical basis for further in-depth research on the function of Mi-UGT79B1 and MpUGT79B1 genes in the petal coloration of Meconopsis and its molecular breeding of flower color.
关键词
全缘叶绿绒蒿/红花绿绒蒿/UGT79B/基因克隆/花色Key words
Meconopsis integrifolia/Meconopsis punicea/UGT79B/Gene cloning/Flower color引用本文复制引用
基金项目
国家自然科学基金(32160404)
国家自然科学基金(31460218)
云南省万人计划青年拔尖人才项目(YNWR-QNBJ-2019-211)
出版年
2024
国家科技期刊平台
NSTL
万方数据