Cloning and Expression Analysis of MiUGT79B1 and MpUGT79B1 Genes from Two Varieties of Meconopsis
To investigate the sequence characteristics of uridine diphosphate glycosyltransferase in different flower color of Meconopsis and its relationship with the flower color of Meconopsis.The yellow-flowered Meconopsis integrifolia and the red-flowered M.punicea were used as study materials,and one UGT gene was screened from the full-length transcriptome data of each of the two flower colors of Meconopsis.Based on the results of KEGG an-notations of the transcriptomes,these two genes were named MiUGT79B1 and MpUGT79B1,and they were cloned,and analyzed by bioinformatic and RT-qPCR.The results showed that the ORF length of MiUGT79B1 and MpUGT79B1 genes was 1 314,encoding 437 amino acids.Both MiUGT79B1 and MpUGT79B1 proteins were hy-drophilic stabilizing proteins with their structures consisting mainly of α-helices and random coil.Phylogenetic tree and multiple sequence comparison analyses revealed that MiUGT79B1 and MpUGT79B1 were most closely related to Papaver somniferum,P.californicum,P.nudicaule,and Macleaya cordata.RT-qPCR analysis showed that Mi-U GT79 B1 and MpUGT79B1 were highly expressed in the bud stage,MiUGT79B1 expression tended to decrease first and then increase,and MpUGT79B1 expression tended to decrease first and then remain stable.There was a significant positive correlation between the expression level of UGT gene and the accumulation pattern of total fla-vonoids.The results of this study provide a theoretical basis for further in-depth research on the function of Mi-UGT79B1 and MpUGT79B1 genes in the petal coloration of Meconopsis and its molecular breeding of flower color.
Meconopsis integrifoliaMeconopsis puniceaUGT79BGene cloningFlower color
国家科技期刊平台
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