Effects of Epbrassinolide on Physiology,Yield and LHcb Gene Expression of Potato under Shading Treatment
In order to investigate how to alleviate the effects of insufficient light in potato production,the potato variety Dongnong 310 was used as the test material,and three shading treatments were set up:normal light(Z0,blackout rate is 0),single-layer blackout screen covering treatment(Z1),double-layer blackout screen covering treatment(Z2).Different concentrations of epbrassinolide(0 mg/L,denoted as CK)were sprayed in the potato tuber formation stage(0.02 mg/L,denoted as E1;0.10 mg/L,labeled E2).Three measuring periods were set,the plant height,chlorophyll a,b content,photosynthetic parameters,fluorescence kinetic parameters,and yield per plant were measured and analyzed.The relative expression levels of LHcb genes in 12 leaves were measured.The results showed that Z1 conditions were more suitable for the growth of Dongnong 310,while Z0 conditions produced photoin-hibition,and the indexes were lower than Z1.Under the condition of Z2,the illumination was insufficient,and the in-dexes were lower than that of Z1.EBR spray could effectively increase the yield per plant,and E2 treatment under Z0 condition significantly increased by 32.62％compared with CK,E1 and E2 treatment under Z1 condition significantly increased by 48.38％and 24.24％compared with CK,respectively,E2 treatment under Z2 condition significantly in-creased by 18.45％compared with CK.In terms of weight of dry matter per tuber,E2 treatment under Z0 condition increased by 27.24％compared with CK,and E1 and E2 treatment under Z,condition increased by 40.09％and 26.56％compared with CK,and the above differences were significant.The relative expression of 12 LHcb genes could be increased by increasing the shading degree and spraying EBR,which was conducive to improving the light energy capture and utilization in leaves.
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