Transcriptome Analysis of Biocontrol Bacteria Bacillus tequilensis Treated Camellia oleifera in Different Ways
To explore the transcriptome changes after biocontrol bacteria treat Camellia oleifera in different ways,and understand the molecular regulatory mechanism of biocontrol bacteria enhancing C.oleifera resistance.The 2-year-old C.oleifera seedlings were selected as experimental materials,and the biocontrol bacterium Bacillus tequilensis was inoculated onto seedlings by leaf spraying and root irrigation,as well as an equal amount of sterile water was used as a control,and the C.oleifera leaves were taken after 15 days of treatment for transcriptome se-quencing analysis.The results indicated that a total of 167 118 913 reads were obtained by transcriptome sequen-cing,with 19 164 differentially expressed genes(DEGs).Among which there were 4 722 DEGs in sample CK-vs-DZY 6715 and 14 442 DEGs in sample CKG-vs-DZY 6715G,and the number of DEGs common to the two groups of samples was 2 573,with the highest number of unique DEGs to sample CKG-vs-DZY 6715G(11 869),followed by sample CK-vs-DZY 6715(2 149).GO enrichment analysis showed that the DEGs of the two groups of samples were annotated into biological processes,molecular functions,and cellular components,mainly involving metabolic process,cellular process,single biological process,cell,cell part,membrane,binding,catalytic activity,transport ac-tivity,etc.KEGG pathway analysis showed that the DEGs of the two groups of samples were enriched into metabolic pathway and biosynthesis of secondary metabolites,while DEGs were also mainly enriched to plant hormone signa-ling transduction in sample CK-vs-DZY 6715,and the DEGs of sample CKG-vs-DZY 6715G were mainly enriched in the plant pathogen interaction.The main DEGs related to disease resistance were up-regulated expression,inclu-ding pckA,PGAM,ACOX1,FATB,asnB,ansA,FLS,LAR,AUX1,GID1,ETR,STPK,CDPK,pfkA.However,there were differences in gene expression levels between spray and root irrigation methods,the expression levels of genes related to the biosynthesis of secondary metabolites and plant hormone signal transduction were higher in spray,while those related to metabolic pathway and plant pathogen interactions were higher in root irrigation.Thus,this pa-per concluded that the biocontrol bacteria Bacillus tequilensis could enhance disease resistance in C.oleifera after treatment with leaf spray or root irrigation,only that the main mechanism of action of the two treatment methods was slightly different.
国家科技期刊平台
NSTL
万方数据
