Effect of Ezrin on Oocyte Maturation and Early Embryonic Development in Mice
The aim of this study was to investigate the effect of Ezrin on the maturation of mouse oocytes,ferti-lization,and early embryo development,as well as to reveal its mechanisms.Mouse germinal vesicle(GV)stage oo-cytes and pronuclear-stage embryos were injected with siRNA targeting Ezrin(si-Ez)or a negative control siRNA(si-NC),then the rates of oocyte maturation,fertilization,and early embryo development were calculated,the length and density of microvilli on the surface of the MⅡ stage oocytes were detected with scanning electron microscopy,the spindle localization and cortical granule migration were observed using immunofluorescence staining.The results showed that knocking down Ezrin during the GV stage significantly reduced the maturation rate of mouse oocytes and extremely significantly lowered the developmental rate of early-stage embryos after fertilization.While it de-creased the fertilization rate of mature oocytes,the difference was not significant.Knocking down Ezrin during the pronuclear stage extremely significantly reduced the total cell number of mouse blastocysts.Although there was a downward trend in the rates of morula and blastocyst survival,there was no significant difference compared to the control group.Scanning electron microscopy revealed that knocking down Ezrin in GV stage mouse oocytes significant-ly decreased the length and density of microvilli on the surface of MⅡ stage oocytes.Immunofluorescence results showed that knocking down Ezrin in GV stage mouse oocytes affected the migration of the spindle and cortical gran-ules.These results suggest that Ezrin,through its influence on microvilli formation,spindle localization,and cortical gran-ule migration,blocks the maturation of mouse oocytes,consequently affecting fertilization and early embryo development.
国家科技期刊平台
NSTL
万方数据
