Cloning and Stress-Resistance Functional Analysis of Calcium-Dependent Protein Kinase Gene TaCDPK17 in Wheat
To investigate the role of calcium-dependent protein kinase(CDPK)in wheat growth and stress re-sponse,the TaCDPK17 gene was cloned from common wheat and its sequence structure,expression pattern,and stress resistance function were preliminarily analyzed.The results showed that the length of the TaCDPK17 gene coding region was 1 701 bp,encoding 566 amino acids and possessing typical structural features of the CDPK fami-ly,including one conserved serine/threonine kinase domain and four EF hand shaped domains.Evolutionary tree analysis of TaCDPK17 and CDPK17 from 12 other plants showed that TaCDPK17 had high homology with the CD-PK17 sequence of gramineous crops,especially Aegilops tauschii and barley.The promoter region of TaCDPK17 gene contained multiple cis regulatory elements related to hormone signaling pathways,light response.Among them,there are more abscisic acid(ABA)responsive elements(ABRE)and methyl jasmonate responsive elements(CGT-CA).The expression analysis based on Real-time Fluorescence Quantitative PCR showed that the expression level of TaCDPK17 increased to varying degrees after induced by 100 μmol/L ABA,100 μmol/L methyl jasmonate,20％PEG6000,and 250 mmol/L NaCl.Under stress conditions of 2 μmol/L ABA and 100 mmol/L NaCl,the germination rate of Arabidopsis seeds overexpressing TaCDPK17 was significantly higher than that of the wild type.Meanwhile,overexpression of TaCDPK17 alleviated the inhibitory effects of ABA or osmotic stress treatments on seedling root growth.During stomatal closure,transgenic plants overexpressing TaCDPK17 are more sensitive to ABA and exhibit a stronger stomatal closure trend compared to wild-type plants.These results indicated that TaCD-PK17 plays an important role in stress response and hormone signaling in wheat.
WheatCalcium-dependent protein kinaseGene cloningExpression analysisStress-resistance
万方数据
维普
