Cloning and Expression Patterns of StRac5,StRac7 and StRac13 Genes in Potato
To explore the expression patterns of potato genes and provide theoretical basis for the subsequent study of gene resistance to potato diseases.Three endogenous small G protein genes StRac5,StRac7 and StRac13 were cloned from potato,and their bioinformatics and expression patterns were analyzed.The results showed that St-Rac5,StRac7 and StRac13 contained similar conserved sequences to the small G proteins in Arabidopsis and rice,all belonging to ROP proteins.The protein domains of StRac5 and StRac13 were identical with AtRop1,AtRop3,AtRop5,AtRop6 of Arabidopsis and rice OsRac5,OsRac6,OsRac7,and StRac7 was identical with AtRop9.Phylogenetic tree a-nalysis showed that StRac7 belonged to class Ⅱ and was closest to Arabidopsis AtRop9.StRac13 belonged to class Ⅲand was the closest genetic relative to Arabidopsis AtRop7.StRac5 belonged to class IV and was the closest genetic relative to rice OsRac5 and OsRac6.The relative expression levels of StRac5,StRac7 and StRac13 genes in different tissues of potato were leaf＞root＞stem.Compared with the expression in stems,the expression of StRac5,StRac7 and StRac1 3 in leaves were increased by 1 222.4％,2 531.3％and 468.2％,respectively.After inoculation,the relative expression of StRac5,StRac7 and StRac1 3 genes increased first and then decreased,and the relative expres-sion of StRac5 and StRac1 3 genes increased by 62.2％,40.4％,respectively,compared with 0 h after inoculation 24 h.The relative expression of StRac7 gene was increased by 827.8％at 72 h after inoculation compared with 0 h.After ABA,SA,GA and 6-BA treatment,the relative expression of StRac5,StRac7 and StRac1 3 genes showed a downward trend.After JA and IAA treatment,the relative expression of StRac5,StRac7 and StRac13 genes showed an up-regulated trend.Therefore,StRac5,StRac7 and StRac13 genes can respond to potato late blight infection,and their expression patterns are different in different tissues and under different hormone treatments.
PotatoSmall G proteinGene cloningLate blightExpression pattern
万方数据
维普
