Mining Candidate Genes of Rind Hardness in Watermelon by BSA-seq and RNA-seq
In order to identify the key genes controlling rind hardness and breed crack-resistant watermelon va-rieties.An F2 segregating population was created using the high-firmness line 901 and the low-firmness line BSH.Both BSA-seq and RNA-seq approaches were utilized to map the genes responsible for rind hardness.The results of BSA-seq revealed an interval region of 2.14 Mb on chromosome 10,spanning from 1 620 000 to 3 760 000,where the intersection of SNPs and InDels identified 150 candidate genes.Among these,two genes showed non-synonymous mutations,and one gene exhibited a frameshift mutation.Correlation analysis between BSA-seq and RNA-seq identi-fied 6 correlated genes,including Cla97C10G187120,Cla97C10G187020,Cla97C10G187430,Cla97C10G187510,Cla97 C10G187280,and Cla97C10G186540.Through bioinformatics analysis,the candidate gene Cla97C10G1 87120 was identified.The result of qRT-PCR indicated that the transcriptome data was reliable.And the relative expression of the candidate gene Cla97C10G187120 was lower in the line 901 than the line BSH.This study lays a crucial foundation for understanding the molecular mechanisms underlying watermelon rind hardness.
万方数据
维普
