Cloning,Subcellular Localization and Expression Analysis of PpLAC7 Gene from Jinxiu Yellow Peach
To clone the PpLAC7 gene sequence of Jinxiu yellow peach,investigating its gene sequence informa-tion and its role in flesh browning.The cDNA sequence of PpLAC7 was cloned from Jinxiu yellow peach by homolo-gous cloning.The promoter region elements,physicochemical properties,secondary and tertiary structure,phyloge-netic tree and amino acid sequence alignment of PpLAC7 gene were performed bioinformatics by using bioinformat-ics software.In addition,it also investigated the subcellular localization and the expression pattern of PpLAC7 during the process of fresh browning in Jinxiu yellow peach.The results of bioinformatics analysis indicated that the promot-er region included light responsiveness,MeJA-responsiveness,drought-inducibility,seed-specific regulation ele-ments,etc.The full length of PpLAC7 gene was 1 692 bp,encoding 563 amino acids,molecular weight was 61.867 ku,total number of atoms was 8 621,GRAVY was-0.028,theoretical pI was 5.95,instability index was 36.48,aliphatic index was 87.26.The secondary structure of PpLAC7 protein mainly contained α-helix of 14.74％,extended strand of 28.77％,β-turn of 6.22％and random coil of 50.27％.Phylogenetic analysis showed that PpLAC7 had high similarity to MdLAC7.Deduced amino acid sequence indicated that PpLAC7 protein,the same as other species,also contained three typical copper ion domains.Subcellular localization analysis revealed that PpLAC7 was located in endoplasmic reticulum.The expression of PpLAC7 gene was up-regulated during the storage of Jinxiu yellow peach fruit.Combining the relationship of flesh browning index and PpLAC7 expression,it indicated that PpLAC7 may play an important role in the browning process of Jinxiu yellow peach fruit.
万方数据
维普
