The aim of this study was to investigate the correlation of single nucleotide polymorphisms of the carboxyl ester lipase gene(CEL)with eye muscle area in Hu sheep.The 1 049 Hu sheep with accurate phenotypic records and in good health were selected,their eye muscle area was determined after slaughter,and blood was col-lected for genomic DNA extraction.Amplification of target fragments and genotyping of CEL gene by PCR and KAS-Par SNP typing,and association analysis with eye muscle area of Hu sheep,RT-qPCR was used to detect the expres-sion of CEL gene in 10 tissues of Hu sheep.The results showed that the Hu sheep CEL gene was expressed in differ-ent tissues and was highest in the duodenum.There was a synonymous mutation of g.4039718 C＞T in this gene,which was moderately polymorphic.Trait association analysis showed that this polymorphic locus was significantly associated with eye muscle area in Hu sheep,with TT-type individuals having significantly higher eye muscle area than CC-type individuals.Descriptive statistics showed that the coefficient of variation for eye muscle area was 12.29％,which had high selection potential.Correlation analysis showed that eye muscle area was significantly and positively correlated with growth and slaughter traits such as body weight,body height,breast circumference,slaugh-ter rate,carcass weight and live weight before slaughter.The results indicated that the g.4039718 C＞T polymorphic locus could be used as a candidate molecular marker for genetic improvement of eye muscle area traits in Hu sheep.
关键词
湖羊/羧基酯脂肪酶/眼肌面积/关联分析
Key words
Hu sheep/Carboxyl ester lipase(CEL)/Eye muscle area/Association analysis
维普
万方数据