The Modulating Influence of Soybeans with High and Low Nodule Density on Their Rhizosphere Microbiota
This study investigates the influence of high and low nodule density on the composition and func-tionality of the soybean rhizosphere microbiome.The study used the wild-type soybean Williams 82 and a high-nodu-lation mutant(HiN)derived from this line to assess the impact of nodule variation on associated microbial popula-tions.A controlled pot experiment was conducted,comparing the growth of both genotypes at the flowering stage,fol-lowed by the collection and high-throughput sequencing of 16S rDNA from the rhizosphere microbes.The high-nodu-lation mutant exhibited significant increases in plant height,aboveground biomass,and nodule count,with respective enhancements of 41.22％,37.46％,and 119.23％over the wild type.Comparative analyses revealed distinct differences in microbial community structure and composition between HiN and WT,with an elevated relative abun-dance of Bacteroidetes in the HiN rhizosphere.At the genus level,several key genera,including Allorhizobium-Neo-rhizobium-Pararhizobium-Rhizobium,Bradyrhizobium,Chryseobacterium,Microbacterium,and Nocardioides,were more abundant in HiN.LEfSe analysis highlighted the enrichment of Actinobacteria and Bacteroidetes in HiN,con-trasting with the predominance of Firmicutes in WT.GUS-labeled Bradyrhizobium further substantiated the enhanced capacity of HiN to enrich Bradyrhizobium.Collectively,these findings elucidate the regulatory role of nodule number on the symbiotic interactions within the soybean rhizosphere microbiome.
SoybeanRoot noduleRhizosphere soilMicrobial community
万方数据
维普
