Efficient shoots regeneration from adventitious buds of cutting chrysanthemum
Using leaf disks of cutting chrysanthemum ( “Jinba” and “Chushui Furong”) as explants, an efficient regeneration system from the adventitious buds was established. The resuits indicated that the genotypes and plant growth regulators greatly affected shoot regeneration from adventitious buds. High concentrations of the plant growth regulators increased the vitrification of adventitious buds and shoots from leaves, and then, decreased the shoot regeneration. The optimum medium which could directly induce shoot regeneration of “Jinba” was MS medium supplemented with 1.0 mg/L naphthalene-acetic acid (NAA) and 1.0 mg/L 6benzylamino purine (6-BA), on which the regeneration rate was 95.5％. In “Chushui Furong”, that was MS medium with 0. 1 mg/L NAA and 1.0 mg/L 6-BA, and the regeneration rate was 46.3％. The plant growth regulators affected the No. of roots induction from shoots obviously, and the optimum rooting medium was 1/2 MS medium with 0.7 mg/L NAA.
NETL
NSTL
万方数据
维普
