Optimization of fermentation conditions and the preperation of tablets for Bacillus subtilis strain B1514
In order to improve the production of Bacillus subtilis strain B1514,reduce production cost and make it convenient for application,single factor and orthogonal experimental designs were carried out to optimize the fermentation conditions and the proportion of carriers,and the preperation conditions of tablets for B1514 were optimized by screening the mesh for wet granulation and the dosage of lubricant magnesium stearate.The results showed that the optimum fermentation medium components for B.subtilis strain B1514 were glucose 1.5％,amylon0.5％,corn flour 3％,MnSO4 0.2％,(NH4)2SO4 1.5％,MgSO4 0.02％,KH2PO4 0.03 ％ and CaCl2 0.1％,respectively; the optimum fermentation conditions were that putting 5％ initial inoculums into 100 mL liquid culture in a 500 mL flask,with the initial pH 7.0-7.5 and keeping the fermentation temperature at 32 ℃.Under the optimum conditions,the number of live bacterium increased to 1.17 × 1010 cfu/mL,and the yield of fermentation increased by 115％.Using corn flour,corn stalk powder,and wheat bran(mass ratio 1 ∶ 6 ∶ 2) as carriers,20 oculus screen for wet granulation and 1％ magnesium stearate as lubricant,can get tablets of B1514 up to standard with intact shape and suitable rigidity.
NETL
NSTL
万方数据
维普
