Isolation, identification, and herbicidal activity of Bacillus cereus XG1
For screening out microbial strains with high herbicidal activity and purifying active substances produced by the strain metabolites,our test isolated microbial strains from the watermelon leaves infected by watermelon bacterial fruit rot,using cauline leaf daub method to measure the herbicidal activity of the strains' second metabolite.The 16S rDNA sequence and physicochemical tests were used to identify the strain having the highest herbicidal activity,and the second metabolites were isolated and purified by silica gel column chromatography combined with high performance liquid chromatography (HPLC) method.The results indicated that 4 bacterial strains with herbicidal activity were isolated,and the strain XG1 had the highest activity on Digitaria sanguinalis,so it was identified as Bacillus cereus.The substance Ⅰ was isolated from fermented liquid by liquid-liquid extraction,silica gel column chromatography and HPLC purification,with the retention time of 15.757 min.The research concluded that the strain XG1 had the potential to be developed as a microbe-herbicide,and the experiment laid a foundation for purifying active substances produced by the second metabolites and developing a new bacterial herbicide.
Bacillus cereusherbicidal activity substanceHPLCisolation and purification
NETL
NSTL
万方数据
维普
