Function of ZmBT2b gene in maize resistance to Fusarium graminearum
In order to investigate the function of the maize BTB-TAZ protein ZmBT2b in maize resistance to pathogenic fungal infection,the ZmBT2b gene was systematically analyzed using bioinformatics techniques in this study.It was determined that ZmBT2b has both BTB and TAZ structural domains.It showed obvious tissue specific expression and different expression patterns under biotic and abiotic stresses.By constructing ZmBT2b gene overexpressing Arabidopsis thaliana plants ZmBT2b-OE,the function of this gene in the process of plant disease resistance was investigated.ZmBT2b-OE plants showed significantly enhanced resistance to Botrytis cinerea and Pst.DC3000,suggesting its function in the process of plant disease resistance.In order to clarify the function of ZmBT2b gene in the process of maize resistance to Fusarium graminearum infestation,maize mutants Zmbt2b-1 and Zmbt2b-2 of this gene were obtained whose disease resistance of were tested.It was found that the F.graminearum resistance was significantly higher in mutants Zmbt2b-1 and Zmbt2b-2 than that of the wild type.The expression of ZmLOXs in JA synthesis pathway and ZmPRs as disease course-related genes were significantly down-regulated during F.graminearum infestation,indicating that ZmBT2b has a positive regulatory function in maize resistance to F.graminearum infestation.The results laid the foundation for elucidating the function of maize BTB-TAZ protein and its regulatory mechanism in the process of maize disease resistance,and provided genetic resources for maize disease resistance breeding.
万方数据
维普
