Application of KASP-SNP markers to detecting heading or non-heading traits in Brassica inbred lines
Leafy head was a significant agronomic trait of distinguishing heading type and non-heading type Chinese cabbage.The development of SNP molecular markers provided an auxiliary tool for leafy vegetable breeding,which could improve the efficiency of traditional heading or non-heading type Chinese cabbage,and greatly enhance the accuracy of genetic analysis and the effectiveness of breeding.In this study,206 inbred lines of Brassica accessions,including 148 heading type and 58 non-heading type Chinese cabbages,were subjected respectively to KASP analysis using four sets of primers,including KBr43,KBr111,KBr233 and KBr258.There were significant differences of the KASP results between heading and non-heading Chinese cabbage using primers KBr111 or KBr258.Among the eight haplotypes composed of SNP sites labeled with three primers sets KBr43,KBr111 and KBr258,a relatively high percentage of ATG and ATC haplotypes was found in heading type Chinese cabbage,while non-heading type Chinese cabbage harbored a relatively high percentage of AGG or AGC haplotypes.Therefore,we inferred that heading Chinese cabbage was more inclined towards ATG and ATC haplotypes,with the difference being that non-heading Chinese cabbage was more tended to AGG and AGC haplotypes.
vegetableheading or non-heading traitsBrassicaKASPSNP molecular markers
NETL
NSTL
万方数据
