Characteristics of lignocellulose degradation by thermotolerant bacteria QK in vegetable waste composting
This study explored the degradation characteristics of lignocellulose by adding microorganisms to aerobic composting.Tomato and melon vegetable wastes were used for high temperature aerobic composting with adding 55 °C temperature tolerant bacillus QK and without bacterial agent as the CK group.Samples were collected on 0 day,7 day,15 day and 31 day to analyze lignocellulose content,enzyme activity and microbial diversity.The results showed that the degradation rates of cellulose,hemicellulose and lignin by adding QK were 65.14%,58.28%and 48.51%,which were 8.27%,7.26%and 15.21%higher than those of the control.The addition of QK significantly increased the activities of carboxymethyl cellulase,hemicellulose xylanase,lignin peroxidase,manganese peroxidase and laccase by 22.64%,25.46%,50%,186.25%and 61.70%compared with the control,respectively.The community structure and diversity of bacteria and fungi were changed by the addition of QK.The Shannon index was significantly increased.The relative abundance of Nocardiopsis and Brachybacterium that promoted lignocellulose degradation was increased.While the relative abundance of pathogenic bacteria Corynebacterium and Aerococcus was decreased.The characteristics of heat-resistant bacteria QK showed that it could be used as a good rot-promoting agent for composting.This study provided a theoretical basis for analyzing the degradation and transformation mechanism of lignocellulose.
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