Identification and expression analysis of key enzyme genes in the kynurenine pathway of Fusarium graminearum
In order to identify the KP key gene and its function,the KP key enzyme gene of Fusarium gramineum was systematically analyzed by bioinformatics method.The key KP enzymes were identified in F.gramineum including IDO(indole-2,3-dioxygenase),AFMID(Arylformamidase),KAT(Kynurenine aminotransferase),KMO(Kynurenine monooxygenase),KYN(Kynureninase)and HAO(hydroxyl acid oxidase).Transcriptom data of F.graminearum showed that the key KP enzyme genes FgIDO,FgAFMID,FgKAT,FgKMO,FgKYN and FgHAO of F.graminearum were highly expressed during conidium stage,mycelium growth stage and different stages of maize stalk infection.The results indicated that the KP genes FgIDO,FgAFMID,FgKAT,FgKMO,FgKYN and FgHAO were involved in conidial production,mycelial growth and infection.Then the pattern map of KP and its key enzyme catalytic reaction of F.graminearum was drawn after summarization of the results and the existing literature information,which laid a foundation for elucidating the function and mechanism of KP key enzyme regulating the growth and pathogenicity of F.graminearum.
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