Detection of drug resistance of Escherichia coli in two types of dairy farms in Hebei Province and analysis of multi-locus sequence typing
In order to study the effect of antimicrobial reduction on bacterial resistance,anal swab samples were collected from standard dairy farms and non-standard dairy farms for routine bacterial isolation and identification,whose drug resistance phenotype,genotype detection and multi-locus sequence typing(MLST)were analyzed.To compare the differences in drug resistance phenotypes and genotypes of E.coli in two types of dairy farms.The results showed that 173 strains of E.coli were isolated from 174 anal swab samples that was composed of 84 strains from the standard farm and 89 strains from the non-standard farm,and the overall isolation rate was 99.4%.The drug resistance test showed that there was no significant difference in the drug resistance rate of E.coli in the standard farm and the non-standard farm(P>0.05).A total of 23 isolates of E.coli were multi-resistant,among which most of high resistant strains(≥fivefold-resistant)came from the non-standard farms.There was no significant difference in the detection rate of other drug resistance genes between the two types of dairy farms(P>0.05),except that the detection rate of QnrB gene in the standard farm was higher than that in the non-standard farm(P<0.05).Twenty-five ST types were identified by MLST typing,in which 20 were known ST types and 5 were new ST types.ST109 was the dominant type of E.coli in the standard farm,and ST10 and ST1307 were the dominant types of E.coli in the non-standard farm.There was no correlation between the antimicrobial resistance phenotype,genotype and ST type of E.coli in dairy farms.
NETL
NSTL
万方数据
