Expression changes of genes and proteins in mouse ovarian granulosa cells during reproductive senescence
In order to investigate the effects of aging on the development of Granulosa cells(GCs)in mouse follicles,we analyzed the transcriptomic and proteomic data from healthy female Kunming(KM)mice aged 6 weeks(young group)and 10 months(old group).The results showed that the differentially expressed genes were not highly consistent with the differentially expressed proteins.A total of 95 genes were up-regulated at both mRNA and protein levels,while 22 genes were down-regulated.The expression levels of related genes were verified by qPCR and Western blot.The results showed that the expressions of apoptosis-related genes Ndrg1 and Sting1 in the aging group were significantly increased(P<0.05)compared with the young group.Significant decreases(P<0.05)were found in antioxidant related genes Gpx7,growth factor related genes Gdf9,Bmpr1b and hormone synthesis and secretion related genes Esr2 and Lhcgr.In conclusion,the apoptosis level of follicular granulosa cells increased,the antioxidant function decreased,and the hormone action pathway was damaged after reproductive senescence.
NETL
NSTL
万方数据
