Development of functional molecular markers diagnostic for wheat resistance to Sitodiplosis mosellana
Orange wheat blossom midge(OWBM)(Sitodiplosis mosellana Géhin)is one of the most important pests that seriously threaten wheat yield in China.Identification of the QTLs relating to wheat resistance to OWBM could improve the selection efficiency of resistance to Sitodiplosis mosellana Géhin and achieve aggregation of resistance with other traits.This study developed and designed markers according to the sequence differences within the preliminarily identified 3.4 Mb-interval major QTL(QSm.hbau-4A)of OWBM-resistant,and tested the detection efficiency of the markers among different genetic background materials.The results showed that there were 371 SSR loci and 3 insertion deletions(InDel)within the target interval,based on which 5 polymorphic markers were developed.90％of the typing results of these markers among the F2 individuals matched their OWBM resistance phenotype.Among them,Xcg116 presented more than 80％(80％-88.89％)likelihood of detection of the 4AL insect resistance locus QSm.hbau-4A.The average detection efficiency of Inl in different resistant varieties was about 67％(67.39％-70.97％).In summary,all two markers can be used to screen wheat germplasm resources for OWBM resistance with locus QSm.hbau-4A.
bread wheat(Triticum aestivum L.)orange wheat blossom midge(Sitodiplosis mosellana Géhin)SSRInDelmarker development
万方数据
维普
