The aim of this study was to investigate the imprinting status and epigenetic modification mechanism of bovine PROSER2 gene.The expression of PROSER2 gene was first analyzed in bovine tissues and placenta using quantitative RT-PCR.The direct sequencing of RT-PCR products were analyzed based-on single nucleotide polymorphism(SNP)for the imprinting status of PROSER2 gene.Finally,the methylation status of two CpG islands covered the promoter/exon 1 region and exon 4 regions of PROSER2 gene,was analyzed using bisulfite sequencing.The results showed that the PROSER2 gene was expressed in all the tested tissues including heart,liver,spleen,lung,kidney,brain and placenta.The PROSER2 gene was monoallelally expressed in the bovine heart,liver,spleen,lung,kidney,brain and placenta.PROSER2 gene was a paternally imprinted gene in cattle,which is consistent with its imprinting status in human.A differentially methylated region was found in the CpG island located in the promoter and the exon 1 of the PROSER2 gene in the bovine heart,liver,spleen,lung,kidney,brain and placenta.This suggests suggested that methylation modifications of DNA may be involved in imprinted expression of bovine PROSER2 gene.This study can provide a reference for the further study of the function and imprinting regulation mechanism of bovine PROSER2 gene.
关键词
DNA甲基化/基因组印记/牛/PROSER2基因/表观遗传
Key words
DNA methylation/genomic imprinting/cattle/PROSER2 gene/epigenetic
维普
万方数据