Study on the function of GhSNAT3A in response to drought stress in Gossypium hirsutum
As an indoleamine compound,melatonin has a wide range of effects on regulating plant growth and development and stress resistance.The serotonin N-acetyltransferase encoded by the SNAT gene is a key enzyme in melatonin synthesis and is also involved in the response of melatonin to environmental stresses.There were 52 SNAT homologous genes in Gossypium hirsutum,among which GhSNAT3A and GhSNAT3D had the highest homology with AtSNAT1.In this study,GhSNAT3A was used as an example to analyze the biological function.It was found that GhSNAT3A and GhSNAT3D had the highest expression in cotton leaves,and GhSNAT3A was located in chloroplasts in cells.GhSNAT3A mRNA was induced by drought stress.Silencing GhSNAT3A(TRV2:GhSNAT3A)resulted in decreased melatonin levels in leaves,decreased activities of antioxidant enzymes including superoxide dismutase(SOD),peroxidase(POD),and catalase(CAT),increased accumulation of free radicals(H2O2 and O2-),and decreased drought tolerance of plants.Overexpression of GhSNAT3A in Arabidopsis significantly improved plant drought tolerance.This study revealed that GhSNAT3A plays an important regulatory role in drought resistance of cotton seedlings and has a potential role in variety improvement of drought-tolerance.
NETL
NSTL
万方数据
