Development and assessment of wheat lines resistant to leaf rust by genetic transformation of TaNAC069
Transcription factors are important disease resistance related proteins in plants.The NAC(NAM,ATAF1/2 and CUC)transcription factor is a unique type in plants,which plays an essential role in plant response to both biotic and abiotic stresses.In our previous study,a wheat(Triticum aestivum L.)NAC transcription factor TaNAC069 was found to be involved in resistance to Puccinia triticina Erikss.(Pt).In this study,TaNAC069 was constructed into the overexpression vector pLGY-02.Then,the recombinant vector pLGY-02-TaNAC069 was transformed into susceptible material'JW'by Agrobacterium-mediated genetic transformation,resulting in 11 stable transgenic wheat lines.PCR validation was performed on the T1～T4 generation plants of the TaNAC069 transgenic wheat,confirming that TaNAC069 has been transferred into 11 wheat lines with genetic stability.Quantitative real-time PCR(qPCR)analysis confirmed that the expression of TaNAC069 gene in 11 positive transgenic plants was higher than that in'JW'.The incidence of wheat leaf rust in the TaNAC069 transgenic lines was milder than that of'JW'with lower spore count,indicating that overexpression of the TaNAC069 gene enhanced the wheat resistance ability against wheat leaf rust.All these results suggested that TaNAC069 played a positive role in defense against wheat leaf rust,and it will be useful to discover the mechanism of TaNAC069 in wheat resistance to leaf rust.
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