Effect of Bacillus megaterium on cadmium and arsenic accumulation and transport in wheat seedlings grown in the co-contaminated soil
To investigate the impact of plant growth-promoting rhizobacteria on the availability of heavy metals in soil and their transport and accumulation in wheat plants,the experiments of soil shaking incubation,long-term soil incubation and soil pot culture were conducted to explore the effects of Bacillus megaterium on the availability of cadmium(Cd)and arsenic(As)in the co-contaminated soil and the translocation and accumulation of Cd and As in wheat seedlings of'Jimai22'.The results of soil shaking incubation showed that Bacillus megaterium could immobilize Cd in soil and significantly reduced the concentrations of soil available Cd by 49.9%after 48 h incubation compared with the autoclaved solution of Bacillus megaterium,and the extracellular secretion markedly reduced the concentrations of soil available As by 142.68%.However,the long-term inoculation with Bacillus megaterium had no significant effect on the availability of soil Cd and As.Furthermore,in the system of soil-wheat seedlings,the addition of Bacillus megaterium significantly decreased the accumulation of Cd in wheat roots by 30.51%in the system of soil and wheat seedlings.Additionally,the application of Bacillus megaterium and the autoclaved bacterial solution reduced soil available As in rhizosphere soil by 8.19%,the As bio-accumulation factor from soil to root by 27.87%,and translocation factor of As from root to shoot by 41.18%,respectively.In conclusion,Bacillus megaterium significantly affected the accumulation and transport of Cd and As in the system of co-contaminated soil-wheat seedling,which may be a significance for safe utilization of contaminated farmland and the safe production of wheat with green methods.
Bacillus megateriumcontaminated soil by heavy metalsavailability of Cd and Asplant-microbial interaction
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