Foxtail millet is a short-day plant and an important minor crop in Northern China.With the in-depth study of foxtail millet,more and more transcription factors related to stress have been identified.The NF-Y family is a type of transcription factor that could bind to CCAAT-box in promoters,including three subfamilies NF-YA,NF-YB and NF-YC.In this study,14 genes of the SiNF-YB subfamily were identified in foxtail millet.The subcellular localization prediction showed that most members of SiNF-YB subfamily were localized in nucleus except SiNF-YB8 and SiNF-YB15.Cis-elements analysis revealed that a large number of abiotic stress and light responsive cis-elements were presented in the promoter regions of SiNF-YB members.Haplotype analysis showed that SiNF-YB4 is strongly associated with drought tolerance which including three haplotypes.The haplotype H003 was likely to be the most elite haplotype showing drought tolerant.SiNF-YB4 was strongly associated with heading date,including haplotype H001 and H002.Transcriptome data analysis showed that the expression level of SiNF-YB5 in root was significantly different between drought tolerance and drought sensitive varieties before and after treatment.And the expression level of SiNF-YB4 was significantly different between spring and summer foxtail millet both under long day and short day.The results provided an important theoretical basis for further investigation of SiNF-YB subfamily genes in foxtail millet,and laid a foundation for breeding drought-tolerance millet varieties to adapt to environmental change at heading date.
维普
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