Identification and bioinformatics analysis of the HAK gene family in Cucumis sativus L.
In order to achieve in-depth study of the functions related to the cucumber K+transporter protein gene family KUP/HAK/KT,the members of CsHAK family were identified by whole genome analysis technology,and their evolutionary relationship,gene structure,protein tertiary structure,cis-acting elements and covariance were analyzed by bioinformatics means.The results revealed that the cucumber genome had a total of 15 CsHAK genes,which were unevenly distributed on cucumber chromosomes chr1,chr3,chr4,chr5 and chr7,and the subcellular localization prediction revealed that the CsHAK genes were located at the cytoplasmic membrane.The CsHAK gene family can be divided into three subfamilies.Most of the family members contained 12 conserved motifs and K_trans conserved functional structural domains.And the promoter regions of the genes contained elements that were closely related to hormone regulation,light response,and stress.Two tandem duplication events occurred between CsHAK family members,and the Ks/Ka values were all less than 1,suggesting that their evolution was subject to purifying selection.The results provided a theoretical basis for further in-depth excavation of the functions and mechanisms of the CsHAKs family of genes in cucumber growth and development and response to adversity stresses.
cucumberhigh affinity potassium ion transporter proteingene familygenome-wide identification
万方数据
维普
