Identification of SUS gene family and analysis of its rhythmexpression in Brassica rapa
Sucrose synthase(SUS)is a glycosyltransferase in plants,which is involved in the regulation of sucrose synthesis and transport.Studying the expression of SUS gene family in Chinese cabbage provides an important basis for revealing the molecular regulation mechanism of glucose metabolism.In this study,we used bioinformatics methods to identify SUS gene family members,and analyze their physical and chemical properties,phylogenetic relationship,protein moieties,gene structure and intra(inter-temporal)collinearity in Chinese cabbage.The relative expression levels of SUS family genes were obtained by RNA-seq technique,and the rhythm expression patterns of SUS family genes in Chinese cabbage were analyzed.The results showed that there were 13 SUS genes in Chinese cabbage,all of which contained SUS domain.The SUS gene family of Chinese cabbage can be divided into 3 subfamilies(SUS Ⅰ,SUS Ⅱ,SUS Ⅲ).The number of conserved motifs in SUS Ⅱ subfamily was the highest.Most genes contain 11～14 CDS sequences.According to the collinearity analysis of SUS gene family in Chinese cabbage,4 pairs of genes were involved in large fragment repetition events,which provided the driving force for Chinese cabbage evolution.The inter-species collinearity analysis of the SUS gene family of Chinese cabbage and Arabidopsis thaliana showed that 13 pairs of homologous genes were constructed between the species,among which there were multiple"one-to-many,many-to-one"homologous gene relationships,and 10 gene pairs belonging to the SUS gene family.The functional enrichment of KEGG and GO genes in SUS family was analyzed.We found that the pathways were enriched in starch and sucrose metabolism,sucrose synthase activity pathways,and so on.The rhythm expression pattern of SUS family genes was analyzed,and it was found that BraA10g019840.3C gene expressed rhythmically in BraSUSs gene,while other genes were mostly highly expressed at night.The results of this study can lay a theoretical foundation for the functional analysis of SUSs gene and the regulation mechanism of sucrose metabolism in Chinese cabbage.
Chinese cabbagesucrose synthasegene familycircadian rhythm expressionbioinfor maticsanalysis
万方数据
维普
