Identification of phenotypic character and ISSR molecules of three wild Clematis hybrids
In order to cultivate new Clematis varieties and enrich its cultivation types,three wild Clematis species were used as parents for cross-breeding.We identified the true hybrids of their progenies,and explored the genetic variation patterns and genetic diversity between the progenies and the parents.The results showed that 43 true hybrids were identified in 47 progenies from six hybrid combinations using 12 ISSR primers.The allele number of parents and progenies varied from 1.731 to 1.937,the effective allele number varied from 1.449 to 1.613,the expected heterozygosity varied from 0.273 to 0.352,and the diversity index ranged from 0.417 to 0.527,which indicated that the progenies were rich in genetic variation.Some of the phenotypic characters in the hybrid progenies were influenced by the parents with variable growth types,including an increasing trend in sepal length,sepal width,flower diameter,and leaf blade width,while the rest of the floral and foliar traits were opposite.The combination of phenotypic character and molecular markers can be effectively used for the early authenticity identification of wild Clematis hybrid offspring,shorten the breeding cycle,and provide a theoretical foundation for the selection of new varieties.
万方数据
维普
