基于表面增强拉曼光谱法的5-羟基色氨酸快速检测方法的研究
Research on rapid detection method of 5-hydroxytryptophan based on surface-enhanced Raman spectroscopy
杨帆 1秦丽 2常化仿 3章祥 3李文华 3吴虎 4孙伟2
作者信息
- 1. 河北省食品安全重点实验室,国家市场监管重点实验室(特殊食品监管技术),特殊食品安全与健康河北省工程研究中心,河北省食品检验研究院,河北 石家庄 050227
- 2. 河北省知识产权保护中心,河北 石家庄 050022
- 3. 普拉瑞思科学仪器(河北)有限公司,河北 石家庄 050000
- 4. 张家港市公安局物证签定室,江苏 张家港 215600
- 折叠
摘要
建立了一种基于表面增强拉曼光谱法快速测定食品中5-羟基色氨酸(5-hydroxytrypto-phan,5-HTP)的方法.本方法采用6次平行实验,样品使用乙腈提取,1 M硝酸反萃后与增强试剂混合上机检测,可以观测到良好的增强拉曼信号,线性范围在0～20 mg/kg,检出限为1 mg/kg.使用该方法检测市售的样品,并结合平行实验验证,检测结果和HPLC方法有较高的一致性.该方法具有操作简单、快速、高效的特点.
Abstract
In this paper,a method for the rapid determination of 5-hydroxytryptophan based on surface-enhanced Raman spectroscopy is established,which has the characteristics of simplicity,fastness and efficiency.This method employs six parallel experiments,and the samples are extracted with acetonitrile,back-extracted with 1 M nitric acid and mixed with the enhancement reagent for on-line detection.A good enhanced Raman signal is observed.The linear range is 0～20 mg/kg,and the detection limit is 1 mg/kg.The above method is used to detect commercially available samples,and combined with parallel experimental verification,the detection results are highly consistent with the HPLC method.
关键词
表面增强拉曼光谱法/快速检测/5-羟基色氨酸(5-HTP)Key words
Surface-enhanced Raman spectroscopy/Rapid detection/5-Hydroxytryptophan引用本文复制引用
基金项目
国家市场监督管理总局技术保障专项(2023YJ15)
中央引导地方科技发展资金项目(科技成果转移转化项目)(236Z1708G)
出版年
2024
NETL
NSTL
万方数据