In this paper,a method for the rapid determination of 5-hydroxytryptophan based on surface-enhanced Raman spectroscopy is established,which has the characteristics of simplicity,fastness and efficiency.This method employs six parallel experiments,and the samples are extracted with acetonitrile,back-extracted with 1 M nitric acid and mixed with the enhancement reagent for on-line detection.A good enhanced Raman signal is observed.The linear range is 0～20 mg/kg,and the detection limit is 1 mg/kg.The above method is used to detect commercially available samples,and combined with parallel experimental verification,the detection results are highly consistent with the HPLC method.
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