To improve the thermal stability of industrial neutral protease and enhance its performance and economy in industrial applications,single-factor and response surface methodology experiments were conducted to identify the optimal protective agents for neutral protease in this study.The optimal protectants for neutral protease were found to be 15％o Na2SO4,4％peptone and 90 U/mL of protease,exhibiting a relative enzyme activity of 92.08％after storage at 40 ℃ for 139 h.A temperature-accelerated model for the composite protective agent neutral protease(JW-3-CP)was established through accelerated heating experiments.Model-predicted values at 25 ℃ were validated against experimental measurements,demonstrating good agreement and accuracy.After 90 days of storage,the composite protective agent neutral protease(JW-3-CP)exhibited a relative activity of(93.37±0.56)％and a turbidity of 0.90±0.12.Compared to the un-protected neutral protease,the relative activity was enhanced by 4.57-fold.
维普
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