Because the channel state of 5G metro transmission network will fluctuate to different degrees under interference,it is difficult to guarantee the stability of signal transmission,so the anti-interference transmission method of 5G metropolitan transmission network is proposed.After the sender sends the short test data to each channel in sequence,the Best Channel deter-mines the Best Channel of the current 5G metropolitan transmission network according to the length of stay of each channel;in the anti-interference transmission stage,a frequency modula-tion mechanism is introduced,using the transmission micro grid to use a small frequency set as the domain for the execution of the hopping communication,ensuring that the channels in the frequency concentration are Best Channel,and performing the transmission task with the mini-mum channel number by updating the frequency set.In the test results,the designed transmis-sion method can maintain the stable output of the signal waveform despite the interference sig-nal intervention.