Regulatory Effect of Honeysuckle Polyphenol Extracts on miR-100-5p in Non-Small Cell Lung Cancer NCI-H1299 Cell Invasion and Migration
Objective:To investigate the impact of honeysuckle polyphenol crude extract on the malig-nant biological behavior of non-small cell lung cancer(NSCLC)NCI-H1299 cells and the molecular mecha-nisms involved.Methods:NCI-H1299 cells were divided into NC group,paclitaxel group,and low,medi-um,and high-dose groups of honeysuckle polyphenol crude extract.Additionally,miR-100-5p group,miR-NC group,anti-miR-100-5p + high-dose group,and anti-miR-NC + high-dose group were established.MTT assay was used to detect cell viability,Transwell assay for cell migration and invasion,Western blot for E-Cadherin,N-Cadherin,Vimentin,and Wnt/β-catenin pathway-related protein expression,and real-time fluorescence quantitative PCR(RT-qPCR)for miR-100-5p expression.Results:After treatment with differ-ent concentrations of honeysuckle polyphenol crude extract,NCI-H1299 cell viability decreased,and the number of cell migration and invasion decreased.E-Cadherin and miR-100-5p expression levels increased,while N-Cadherin and Vimentin expression levels decreased(P<0.05).Overexpression of miR-100-5p in-creased NCI-H1299 cell viability,cell migration[(226±20.03)cells],and invasion[(171±15.03)cells],while E-Cadherin expression level decreased(0.34±0.03),and N-Cadherin(0.88±0.08)and Vi-mentin expression levels increased(P<0.05).Downregulating miR-100-5p could reverse the effects of hon-eysuckle polyphenol crude extract on NCI-H1299 cell proliferation,migration,invasion,and the Wnt/β-catenin pathway.Conclusion:Honeysuckle polyphenol crude extract may inhibit the proliferation,migration,and invasion of NSCLC NCI-H1299 cells by upregulating miR-100-5p expression,thereby suppressing the activation of the Wnt/β-catenin signaling pathway.
国家科技期刊平台
NSTL
万方数据
维普
