The Regulatory Effect of LncRNA PURPL on the miR-342-3p/PIK3R1 Axis and its Impact on the Malignant Biological Behavior of Hepatocellular Carcinoma Cells
Objective:To investigate the impact of long non-coding RNA(PURPL)on the miR-342-3p/phosphoinositide-3-kinase regulatory subunit 1(PIK3R1)axis and its effects on the malignant biological behavior of hepatocellular carcinoma(HCC)cells.Methods:Cell Experiments:Si-NC,si-PURPL,si-PURPL+inhibitor NC,si-PURPL+miR-342-3p inhibitor were separately transfected into HCC cells(Huh-7)and named si-NC group,si-PURPL group,si-PURPL+inhibitor NC group,si-PURPL+miR-342-3p inhibitor group,while untreated Huh-7 cells were designated as the NC group.Dual-luciferase reporter gene experiments verified the relationship between LncRNA PURPL,miR-342-3p,and PIK3R1.qRT-PCR was used to detect the expression of LncRNA PURPL and miR-342-3p in human normal liver cells(L02)and Huh-7 cells.Western blot assessed PIK3R1 protein levels in L02 and Huh-7 cells.MTT assay evaluated Huh-7 cell proliferation,flow cytometry measured apoptosis rates,Transwell assay determined invasive cell numbers,and scratch tests assessed cell migration.In Vivo Experiments:A xenograft mouse model was estab-lished with the same cell treatment groups.Tumor volume and weight were measured.Results:Cell experi-ment results showed that compared with the si-NC group,si-PURPL group had significantly decreased OD490 values,scratch healing rates,invasive cell numbers,LncRNA PURPL,and PIK3R1 levels in Huh-7 cells(P<0.05).Apoptosis rates and relative expression of miR-342-3p significantly increased(P<0.05).In-hibiting miR-342-3p expression weakened the inhibitory effects of silencing LncRNA PURPL on Huh-7 cell proliferation,migration,invasion,and promotion of apoptosis.LncRNA PURPL negatively regulated the miR-342-3p/PIK3R1 axis.In vivo results showed that the tumor volume and mass of si-PURPL group mice de-creased compared to the si-NC group(P<0.05).Inhibiting miR-342-3p expression weakened the inhibitory effect of silencing LncRNA PURPL on tumor growth in vivo.Conclusion:Silting LncRNA PURPL can inhibit the malignant biological behavior of Huh-7 cells,and the mechanism may be related to the regulation of miR-342-3p/PIK3R1 axis.
国家科技期刊平台
NSTL
万方数据
维普
