Involvement of miRNA-93-5p in Cervical Cancer Progression by Regulating FBXW7 Gene
Objective:To explore whether miRNA-93-5p can affect the progression of cervical cancer by regulating FBXW7 gene.Methods:Twenty clinical cervical cancer patient tissues were collected and di-vided into cancer-adjacent tissues and cervical cancer tissues.RT-qPCR was used to detect the levels of miRNA-93-5p and FBXW7 mRNA in cervical tissues.The miRNA-93-5p and FBXW7 mRNA levels were also measured in normal cervical epithelial cells(H8)and cervical cancer cells(Caski,HeLa,C33A).The cervical cancer cell line C33A was selected for further experiments.C33A cells were divided into the control group(normal culture),miRNA-93-5p inhibition group(transfected with low-expression miRNA-93-5p),and FBXW7 activation group(transfected with high-expression FBXW7).After 48 hours of transfection,cells were collected,and RT-qPCR was used to detect miRNA-93-5p and FBXW7 mRNA levels.Western blot-ting measured FBXW7 protein levels in C33A cells.Flow cytometry assessed apoptosis rates,plate colony as-says examined cloning ability,scratch tests evaluated migration capacity,and Transwell assays measured inva-sion ability.Results:In comparison with cancer-adjacent tissues,cervical cancer tissues showed increased miRNA-93-5p mRNA levels and decreased FBXW7 mRNA levels(P<0.001).Compared with H8 cells,Caski,HeLa,and C33A cells exhibited increased miRNA-93-5p mRNA levels and decreased FBXW7 mR-NA levels(P<0.05).Subsequent experiments were conducted using the C33A cervical cancer cell line.In the miRNA-93-5p inhibition group,miRNA-93-5p mRNA levels significantly decreased(P<0.001),ap-optosis rates increased(P<0.001),cloning ability decreased(P<0.01),migration distance reduced(P<0.01),invasion ability decreased(P<0.01),and FBXW7 protein and mRNA levels significantly increased(P<0.01)compared with the control group.In the FBXW7 activation group,FBXW7 mRNA levels significantly increased(P<0.01),apoptosis rates increased(P<0.001),cloning ability decreased(P<0.001),migra-tion distance reduced(P<0.01),invasion ability decreased(P<0.01)compared with the control group.Conclusion:miRNA-93-5p can affect the apoptosis,proliferation,migration and invasion of cervical cancer cells by regulating FBXW7 gene.
国家科技期刊平台
NSTL
万方数据
维普
