摘要
目的:探讨肠道菌群代谢相关短链脂肪酸(SCFAs)与6-9 岁健康儿童骨骼肌质量的相关性分析.方法:在2021 年12 月至2023 年1 月期间在本院进行这项横断面研究.总共有 176 名参与者(68 名女孩和108 名男孩)纳入本研究.采用双 X 射线吸收测定法(DXA)测量骨骼肌质量指标,并根据骨矿物质密度(BMD)对参与者进行分层分析.使用高效液相色谱(HPLC)测定粪便中 SCFAs 的浓度.通过Spearman相关分析粪便SCFAs与骨骼肌质量的关系.结果:根据 TBLH 骨密度参考值计算 Z值,纳入研究的儿童被分层为Z得分≥0 的正常BMD(n=120)和Z得分<0 的低BMD(n=56).与正常BMD组相比,低BMD组的TB BMD、TBLH BMD、LS BMD、PELV BMD、TSM/TBF、ASM/AFM均显著降低(P<0.05),和TSM、ASM均显著增加(P<0.05).与正常 BMD 组相比,低 BMD 组的粪便戊酸水平显著增加(P<0.05),和乙酸、丁酸、总SCFAs水平显著降低(P<0.05).校正协变量和 FDR 校正后,多元回归分析显示TBLH BMD、PELV BMD与丁酸、戊酸和总 SCFAs 显著正相关(β= 0.15～0.23,P<0.05).结论:粪便丁酸、戊酸和总SCFAs与6～9 岁健康儿童的BMD呈正相关.
Abstract
Objective:To explore the correlation between gut microbiota metabolites,specifically short-chain fatty acids(SCFAs),and skeletal muscle mass in healthy children aged 6-9.Methods:This cross-sectional study was conducted from December 2021 to January 2023.A total of 176 participants(68 girls and 108 boys)were included in the study.Skeletal muscle mass indices were measured using dual-energy X-ray absorptiometry(DXA),and participants were stratified based on bone mineral density(BMD)values.The concentration of SCFAs in feces was determined using high-performance liquid chromatography(HPLC).Spearman correlation analysis was performed to assess the relationship between fecal SCFAs and skeletal mus-cle mass.Results:Based on TBLH bone density reference values,children included in the study were classi-fied into normal BMD(Z score≥0,n = 120)and low BMD(Z score<0,n = 56)groups.Compared to the normal BMD group,the low BMD group showed significantly lower TB BMD,TBLH BMD,LS BMD,PELV BMD,TSM/TBF,ASM/AFM(P<0.05),and significantly increased TSM,ASM(P<0.05).The low BMD group had a significantly higher level of fecal butyric acid and significantly lower levels of acetic acid,propionic acid,total SCFAs compared to the normal BMD group(P<0.05).After adjusting for covariates and false discovery rate(FDR)correction,multivariate regression analysis showed a significant positive correlation between TBLH BMD,PELV BMD,and butyric acid,propionic acid,and total SCFAs(β=0.15～0.23,P<0.05).Conclusion:Fecal butyric acid,propionic acid,and total SCFAs are positively correlated with bone mineral density(BMD)in healthy children aged 6～9.
国家科技期刊平台
NSTL
维普
万方数据