Impact of miR-145-5p-Mediated Regulation of Survivin via the Apoptotic Pathway on the Biological Behavior of Esophageal Cancer
Objective:To explore the impact of miR-145-5p targeting Survivin and the apoptotic signa-ling pathway on apoptosis,invasion,and migration in esophageal cancer.Methods:Real-time quantitative polymerase chain reaction(RT-qPCR)was employed to detect the relative expression levels of miR-145-5p and Survivin mRNA in esophageal cancer tissues and cell lines.A dual-luciferase reporter gene analysis was conducted to validate the targeted regulatory relationship between miR-145-5p and Survivin.RT-qPCR was used to measure the relative expression levels of miR-145-5p and Survivin mRNA after cell transfection.Western blot was performed to assess the expression of BCL2,MDM2,and Caspase-3 proteins in the apoptot-ic pathway.Cell survival,apoptosis rate,migration rate,and invasion ability were evaluated using MTT,flow cytometry,scratch healing,and Transwell chamber experiments in TE-1 cells.Results:Compared to adjacent normal tissues,miR-145-5p was downregulated,while Survivin was upregulated in cancer tissues(P<0.05).In comparison to normal esophageal mucosal epithelial HEEC cells,TE-1 cells exhibited low expression of miR-145-5p and high expression of Survivin(P<0.05).Dual-luciferase reporter gene experiments con-firmed the targeted regulatory relationship between miR-145-5p and Survivin.Transfection with miR-145-5p mimic significantly increased miR-145-5p expression,inhibited Survivin mRNA expression,downregulat-ed BCL2 and MDM2 protein expression,upregulated Caspase-3 expression,promoted cell apoptosis,and in-hibited cell viability,invasion,and migration compared to untransfected and miR-145-5p control-transfected TE-1 cells(P<0.05).Conclusion:Through negative regulation by miR-145-5p,the expression of Sur-vivin is inhibited,leading to the activation of the apoptotic pathway.This promotes cell apoptosis and inhibits the survival,invasion,and migration capabilities of esophageal cancer cells.
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维普
