Impacts of Polysaccharide of Trametes Robiniophila Murr on the Malignant Biological Behavior of Cervical Cancer Cells by Regulating the SPHK1/S1P/S1PR3 Signaling Pathway
Objective:To investigate the impacts of Huaier polysaccharide(HP)regulating the SPHK1/S1P/S1PR3 signaling pathway on the malignant biological behavior of cervical cancer cells.Meth-ods:MTT was used to detect the viability of cervical cancer cells treated with Sophora auricula polysaccharides(0,25,50,100,200,400μg/mL)and screen for the optimal drug concentration.The subjects were divided into control group,low,medium,and high concentration groups of Huaier polysaccharide(HP-L,HP-M,HP-H groups),and high concentration of Huaier polysaccharide+SphK1 activator K6PC-5 group(HP-H+ K6PC-5 group).The cell proliferation,migration and invasion were observed;Western blot was used to de-tect the protein levels of SPHK1,S1P,S1PR3,Snail,N-cadherin,and E-cadherin.Results:After treat-ment for 24,48 and 72h,HP treatments of 50μg/mL,100μg/mL,200μg/mL and 400μg/mL significantly decreased cell viability compared with 0μg/mL(P<0.05),Edu positive rate,number of invasive cells,scratch healing rate and Snail,N-cadherin,SPHK1,S1P and S1PR3 levels in HP-L,HP-M and HP-H groups were obviously lower than those in Control group(P<0.05),E-cadherin levels significantly increase(P<0.05).The Edu positive rate,number of invasive cells,scratch healing rate and Snail,N-cadherin,SPHK1,S1P and S1PR3 levels in HP-H+K6PC-5 group were obviously higher than those in HP-H group(P<0.05),E-cadherin levels significantly decrease(P<0.05).Conclusion:HP may inhibit the prolifera-tion,invasion and migration of cervical cancer cells by inhibiting SPHK1/S1P/S1PR3 signaling pathway.
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