Effect of miR-1297 on Regulating Invasion and Migration of Gastric Cancer Cells via HMGA1 Gene
Objective:To investigate whether miR-1297 regulates the migration and invasion of gastric cancer by targeting high mobility group A1(HMGA1).Methods:The expression levels of miR-1297 and HMGA1 in gastric cancer(n=30)and adjacent normal tissues were determined by RT-qPCR.Gastric adeno-carcinoma SGC-7901 cell line and human normal gastric mucosal epithelial cells GES-1 were cultured in vitro.SGC-7901 cells were divided into blank group,negative control group,miR-1297 mimics group,miR-1297 inhibitor and miR-1297 inhibitor+HMGA1 siRNA group.Transwell method was used to detect the mi-gration and invasion of SGC-7901 cells.Western blot tests for changes in protein levels.The specific target genes of miR-1297 were detected by luciferase reporter gene assay.Results:The expression of miR-1297 was down-regulated in gastric cancer tissues and SGC-7901 cells,and HMGA1 mRNA level was increased at the same time(P<0.001),and miR-1297 and HMGA1 mRNA expression were negatively correlated(P<0.05).Compared with blank group and negative control group,miR-1297 mimics group could inhibit the mi-gration and invasion ability of cells in vitro(P<0.05).In addition,miR-1297 down-regulates HMGA1 by targeting its 3'-UTR.Compared with blank and negative controls,transfection with miR-1297 inhibitor after downregulation of miR-1297 expression significantly increased cell migration and invasion ability(P<0.05).At the same time,the promoting ability was reversed after transfection of HMGA1 siRNA(miR-1297 inhibitor+HMGA1 siRNA group)(P<0.05).Conclusion:miR-1297 inhibits the migration and invasion of SGC-7901 cells by targeting HMGA1,suggesting that miR-1297/HMGA1 axis provides a new prospective thera-peutic strategy for gastric cancer.
万方数据
维普
