Static Magnetic Field Promotes the Proliferation Activity and Migration Phenotype of Skin Fibroblasts through Activation of the FGFR1/JAK2/STAT3 Signaling Pathway
Objective:To investigate the regulatory effects of static magnetic field(SMF)on the prolif-eration activity and migration phenotype of skin fibroblasts and its potential mechanism.Methods:Human skin fibroblasts(HSFs)were divided into five groups:control group,SMF group,SMF+AG group,SMF+LCK group,and SMF+Stattic group.The control group was HSFs cultured normally.HSFs cells in the SMF group were exposed to 1mT SMF.The other three groups were pre-incubated with inhibitors of FGFR1,JAK2,and STAT3(3 μmoL/L AG,3 nmoL/L LCK,and 20 μmoL/L Stattic)together with HSFs and exposed to 1 mT SMF.All groups were treated for 24 hours.Cell Counting Kit-8(CCK-8)was used to analyze cell prolifera-tion activity.ELISA was used to detect the level of Bcl-2-associated X protein(BAX).Western blot was used to detect the expression of apoptotic marker cleaved-caspase3 and FGFR1,JAK2,STAT3,phosphoryla-ted(p)-FGFR1,p-JAK2,p-STAT3,and the expression of cell migration-related phenotypes high-mobility group box 1(HMGB1),vimentin,vascular endothelial growth factor A(VEGFA),matrix metallopeptidase-9(MMP-9),and MMP-2.Results:Compared with the control group,the cell proliferation activity in the SMF group was increased,the BAX level was decreased,the protein expression level of cleaved-caspase3 was down-regulated,and the expression levels of p-FGFR1,p-JAK2,p-STAT3,HMGB1,vimentin,VEGFA,MMP-9,and MMP-2 were all significantly up-regulated(all P<0.05).Compared with the SMF group,the cell proliferation activity in the SMF+AG group was decreased,the BAX level was increased,the protein ex-pression level of cleaved-caspase3 was up-regulated,and the expression levels of p-FGFR1,p-JAK2,p-STAT3,HMGB1,vimentin,VEGFA,MMP-9,and MMP-2 were all significantly down-regulated(all P<0.05).Compared with the SMF group,the cell proliferation activity in the SMF+LCK group was decreased,the BAX level was increased,the protein expression level of cleaved-caspase3 was up-regulated,and the expres-sion levels of JAK2,p-JAK2,p-STAT3,HMGB1,vimentin,VEGFA,MMP-9,and MMP-2 were all sig-nificantly down-regulated(all P<0.05).Compared with the SMF group,the cell proliferation activity in the SMF+Stattic group was decreased,the BAX level was increased,the protein expression level of cleaved-caspase3 was up-regulated,and the expression levels of STAT3,p-STAT3,HMGB1,vimentin,VEGFA,MMP-9,and MMP-2 were all significantly down-regulated(all P<0.05).Conclusion:SMF promotes the proliferation activity and migration phenotype of skin fibroblasts by activating the FGFR1/JAK2/STAT3 signa-ling pathway.
Static magnetic fieldSkin fibroblastsFGFR1/JAK2/STAT3 signaling pathwayProliferationMigration
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