Objective:To investigate the effect of active vitamin D3(VD3)on the characteristics of re-nal mesangial cell fibrosis induced by high glucose,and to explore the relevant mechanism.Methods:Rat mesangial cell line HBZY-1 was cultured and divided into normal glucose culture(NG)group,normal glu-cose combined with VD3 culture(NG+VD3)group,high glucose culture(HG)group,high glucose com-bined with VD3 culture(HG+VD3)group,high glucose combined with n-acetylcysteine culture(HG+NAC)group.The proliferation activity of cells in each group was detected by CCK-8 method,the apoptosis rate of cells in each group was detected by flow cytometry,the content of reactive oxygen species(ROS)in cells in each group was detected by DCFH-DA method,the levels of interleukin-1β(IL-1β),IL-18 and tumor necrosis factor-α(TNF-α)in cell supernatant were detected by enzyme-linked immunosorbent assay(ELISA),the mRNA expression and the protein expression of transforming growth factor-β1(TGF-β1),fi-bronectin(FN),intercellular adhesion molecule-1(ICAM-1),thioredoxin interacting protein(TXNIP),NOD-like receptor heat protein domain associated protein 3(NLRP3)in cells in each group were detected by real-time fluorescent quantitative polymerase reaction(RT-qPCR)and Western blot.Results:Compared with NG group,the proliferative activity in HG group was significantly increased(P<0.05),the apoptosis rate was significantly decreased(P<0.05),ROS content was significantly increased(P<0.05),the levels of IL-1β,IL-18 and TNF-α in cell supernatant were significantly increased(P<0.05),and the mRNA and protein relative expression levels of TGF-β1,FN,ICAM-1,TXNIP and NLRP3 were significantly up-regu-lated(P<0.05).Compared with HG group,the proliferative activity in HG+VD3 and HG+NAC groups was significantly decreased(P<0.05),the apoptosis rate was significantly increased(P<0.05),the ROS content was significantly decreased(P<0.05),the levels of IL-1β,IL-18 and TNF-α in cell supernatant were sig-nificantly decreased(P<0.05),and the mRNA and protein relative expression levels of TGF-β1,FN,ICAM-1,TXNIP and NLRP3 were significantly down-regulated(P<0.05).Conclusion:VD3 can alleviate hy-perglycemia-induced hyperproliferation and fibrosis of renal mesangial cells,which may be related to the regu-lation of ROS/TXNIP/NLRP3 pathway.
Diabetic nephropathyMesangial cells of kidneyActive vitamin D3Prolifera-tionFiber
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