Effect of Combined Ropivacaine and Sufentanil Anesthesia in Mid-Pregnancy on Progeny Brain-Derived Neurotrophic Factor Expression and Cognitive Function in SD Rats
Objective:To investigate the effects of combined ropivacaine and sufentanil anesthesia in mid-pregnancy on progeny brain-derived neurotrophic factor(BDNF)expression and cognitive function in SD rats.Methods:The study hypothesized that maternal treadmill exercise during pregnancy could prevent neuro-toxicity induced by ropivacaine and sufentanil.In the first experiment,pregnant rats were exposed to a 100 μL mixture of sufentanil(0.25 μg/μL)and ropivacaine(0.125 μg/μL)(R&G)on gestational(G)day 14,or were exposed continuously on G13,G14,and G15(R&Gx3).In the second experiment,pregnant rats in the exercise group were forced to run on a treadmill for 60 minutes daily throughout pregnancy.The TrkB antago-nist ANA-12 was used to investigate whether the BDNF/TrkB signaling pathway was involved in the neuropro-tection provided by maternal exercise.Results:Repeated but not single exposure to ropivacaine and sufentanil led to a decrease in histone acetylation and BDNF expression in fetal brain tissue and postnatal hippocampus.Spatial learning and memory impairments were compromised,accompanied by a reduction in dendritic spine number.Maternal exercise mitigated these effects,but the TrkB antagonist ANA-12 abolished the beneficial effects of maternal exercise.Conclusion:Multiple(but not single)exposures to ropivacaine and sufentanil in mid-pregnancy in pregnant rats lead to long-term learning and memory impairments in offspring.Maternal ex-ercise improves postnatal neurocognitive deficits by enhancing histone acetylation and activating downstream BDNF/TrkB signaling.
Mid-pregnancyRopivacaineSufentanilBrain-derived neurotrophic factorCognitive function
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维普
