河北医学2024，Vol.30Issue(5) ：773-781.DOI:10.3969/j.issn.1006-6233.2024.05.013

妊娠中期罗哌卡因联合舒芬太尼麻醉对SD模型子代脑源性神经营养因子表达和认知功能的影响

Effect of Combined Ropivacaine and Sufentanil Anesthesia in Mid-Pregnancy on Progeny Brain-Derived Neurotrophic Factor Expression and Cognitive Function in SD Rats

底妍 李立函 焦姗姗 张坤 郝书航 王金保
河北医学2024，Vol.30Issue(5) ：773-781.DOI:10.3969/j.issn.1006-6233.2024.05.013
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妊娠中期罗哌卡因联合舒芬太尼麻醉对SD模型子代脑源性神经营养因子表达和认知功能的影响

Effect of Combined Ropivacaine and Sufentanil Anesthesia in Mid-Pregnancy on Progeny Brain-Derived Neurotrophic Factor Expression and Cognitive Function in SD Rats

底妍 1李立函 1焦姗姗 1张坤 1郝书航 1王金保1
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作者信息

  • 1. 中国人民解放军联勤保障部队第九八〇医院麻醉科,河北 石家庄 050082
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摘要

目的:探讨妊娠中期罗哌卡因联合舒芬太尼麻醉对 SD 模型子代脑源性神经营养因子表达和认知功能的影响.方法:本研究假设怀孕期间的母体跑步机运动可以防止罗哌卡因联合舒芬太尼引起的神经毒性.在第一个实验中,怀孕大鼠在妊娠(G)第 14 天暴露于舒芬太尼(0.25μg/μL)+罗哌卡因(0.125μg/μL)混合液 100μL(R&G),或在 G13,G14 和 G15 上连续暴露(R&Gx3).在第二个实验中,运动组的怀孕大鼠在整个怀孕期间被迫在跑步机上每天跑 60min.TrkB 拮抗剂 ANA-12 被用来研究脑源性神经营养因子(brain-derived neurotrophic factor,BDNF)/TrkB 信号通路是否参与了母亲运动所提供的神经保护.结果:重复而非单一暴露于罗哌卡因联合舒芬太尼会导致胎儿脑组织和出生后海马中组蛋白乙酰化和BDNF表达的降低.伴随着树突棘数量的减少,空间依赖性学习和记忆功能障碍受损.母体运动减轻了这些影响,但TrkB拮抗剂ANA-12 消除了母体运动的有益影响.结论:妊娠大鼠在妊娠中期多次(而非单次)接触罗哌卡因联合舒芬太尼会导致后代长期学习和记忆功能障碍.母体运动通过增强组蛋白乙酰化和激活下游BDNF/TrkB信号传导以改善产后神经认知障碍.

Abstract

Objective:To investigate the effects of combined ropivacaine and sufentanil anesthesia in mid-pregnancy on progeny brain-derived neurotrophic factor(BDNF)expression and cognitive function in SD rats.Methods:The study hypothesized that maternal treadmill exercise during pregnancy could prevent neuro-toxicity induced by ropivacaine and sufentanil.In the first experiment,pregnant rats were exposed to a 100 μL mixture of sufentanil(0.25 μg/μL)and ropivacaine(0.125 μg/μL)(R&G)on gestational(G)day 14,or were exposed continuously on G13,G14,and G15(R&Gx3).In the second experiment,pregnant rats in the exercise group were forced to run on a treadmill for 60 minutes daily throughout pregnancy.The TrkB antago-nist ANA-12 was used to investigate whether the BDNF/TrkB signaling pathway was involved in the neuropro-tection provided by maternal exercise.Results:Repeated but not single exposure to ropivacaine and sufentanil led to a decrease in histone acetylation and BDNF expression in fetal brain tissue and postnatal hippocampus.Spatial learning and memory impairments were compromised,accompanied by a reduction in dendritic spine number.Maternal exercise mitigated these effects,but the TrkB antagonist ANA-12 abolished the beneficial effects of maternal exercise.Conclusion:Multiple(but not single)exposures to ropivacaine and sufentanil in mid-pregnancy in pregnant rats lead to long-term learning and memory impairments in offspring.Maternal ex-ercise improves postnatal neurocognitive deficits by enhancing histone acetylation and activating downstream BDNF/TrkB signaling.

关键词

妊娠中期/罗哌卡因/舒芬太尼/脑源性神经营养因子/认知功能

Key words

Mid-pregnancy/Ropivacaine/Sufentanil/Brain-derived neurotrophic factor/Cognitive function

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基金项目

河北省医学科学研究课题(2020)(20200258)

出版年

2024
河北医学
河北省医学会

河北医学

CSTPCD
影响因子：1.915
ISSN：1006-6233
参考文献量15
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