Objective:To investigate the influence and significance of 5-aminolevulinic acid photody-namic therapy(ALA-PDT)on the expression of p53 and Caveolin-1 in Bowen's disease(BD)lesions.Methods:Forty cases of Bowen's disease and adjacent normal skin tissues were treated with ALA-PDT.Im-munohistochemical staining(SP method)was used to detect the positive expression rates of p53 and Caveolin-1 in BD and surrounding normal skin tissues before and after photodynamic therapy.Results:Before treat-ment,the positive rates of p53 protein expression in normal skin tissue and BD were 10%and 40%,respec-tively(χ2=11.202,P<0.001),with a statistically significant difference.After treatment,the positive rates of p53 in normal skin tissue and BD were 10%and 5%(χ2=0.712,P=0.399),respectively,with no sta-tistically significant difference between them.The difference in the positive expression rate of p53 in BD before and after treatment was statistically significant(χ2=14.811,P<0.001).Before treatment,the positive ex-pression rates of Caveolin-1 in normal skin tissue and BD were 15%and 55%,respectively(χ2=14.449,P<0.001),with a statistically significant difference.After treatment,the positive rates of Caveolin-1 in normal skin tissue and BD were 5%and 12.5%(χ2=0.816,P=0.366),respectively,with no statistically signifi-cant difference between them.The difference in the positive expression rate of Caveolin-1 in BD before and after treatment was statistically significant(χ2=19.013,P<0.001).There was a positive correlation between p53 and Caveolin-1 expression in BD(r=0.533,P=0.015).Conclusion:The high expression of p53 and Caveolin-1 may be closely related to the occurrence of BD,and ALA-PDT can inhibit the expression of p53 and Caveolin-1,thereby inhibiting the progression of the disease.With a larger sample size,p53 and Caveo-lin-1 may become diagnostic tools for skin-related diseases and provide new targets for their treatment.
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