Objective:To investigate the association between the expression levels of microRNA-1470(miR-1470),POZ domain and AT-binding motif zinc finger protein 1(PATZ1)in hepatocellular carcinoma(HCC)tissues and patient prognosis within 5 years.Methods:A total of 135 patients with HCC who under-went radical resection at Handan Central Hospital from September 2015 to August 2018 were enrolled in this study.HCC tissues and corresponding adjacent normal tissues(normal liver tissues more than 2 cm away from the tumor margin)were collected during surgery.Immunohistochemistry was used to detect PATZ1 protein ex-pression;real-time fluorescence quantitative PCR was used to detect miR-1470 and PATZ1 mRNA expres-sion.Patients were followed up for 5 years,and 5-year overall survival rates were calculated.The expression of miR-1470 and PATZ1 mRNA and protein in HCC tissues and adjacent normal tissues was compared;the relationship between miR-1470 and PATZ1 mRNA expression and clinicopathological parameters,their corre-lation,and their association with prognosis were analyzed;and the risk factors affecting prognosis were identi-fied.Dual luciferase reporter gene assay was used to verify the target relationship between miR-1470 and PATZ1.Results:The positive expression rate of PATZ1 protein in HCC tissues was significantly lower than that in adjacent normal tissues(P<0.05).Compared with adjacent normal tissues,the expression level of miR-1470 was significantly upregulated and the expression level of PATZ1 mRNA was significantly downregu-lated in HCC tissues(P<0.05).Patients with low miR-1470 and high PATZ1 mRNA expression in HCC tis-sues had significant differences in serum alpha-fetoprotein(AFP)level,China Liver Cancer Staging System(CNLC)stage,and the presence or absence of vascular tumor thrombus(P<0.05).The expression of miR-1470 and PATZ1 mRNA was negatively correlated in HCC tissues(P<0.05).The 5-year overall survival rates of patients with low miR-1470 expression and high PATZ1 mRNA expression were significantly higher than those of patients with high miR-1470 expression and low PATZ1 mRNA expression(P<0.05).CNLC stage Ⅲ,vascular tumor thrombus,high miR-1470 expression,and low PATZ1 mRNA expression were inde-pendent risk factors for poor prognosis in HCC patients(P<0.05).miR-1470 could negatively regulate PATZ1 expression.Conclusion:miR-1470 is upregulated and PATZ1 is downregulated in HCC tissues,and the two are negatively correlated.Both can be used as biomarkers to evaluate the prognosis of HCC patients,and miR-1470 can negatively regulate PATZ1 expression.
关键词
肝癌/微小RNA-1470/POZ结构域和AT结合基序锌指蛋白1/预后
Key words
Hepatocellular carcinoma/microRNA-1470/POZ domain and AT-binding zinc fin-ger protein 1/Prognosis
维普
万方数据