circ-RBM33 Targets the miR-383-3p/CKS1B Axis to Inhibit Proliferation and Invasion of Nasopharyngeal Carcinoma Cells
Objective:To investigate whether circ-RBM33 can target the miR-383-3p/CKS1B axis to inhibit the proliferation and invasion of nasopharyngeal carcinoma cells.Methods:qRT-PCR was used to de-tect the expression of circ-RBM33 and miR-383-3p in CNE1 and HNEpC cells.CNE1 cells were randomly divided into si-NC group,si-RBM33 group,miR-NC group,miR-383-3p group,and si-RBM33+anti-miR-383-3p group.Cell proliferation(CCK-8 assay),invasion(Transwell assay),migration(scratch as-say),and expression of CKS1B protein in cells(Western blot)were detected.Luciferase activity assay was performed to determine the targeting relationship between circ-RBM33 and miR-383-3p/CKS1B.Results:The relative expression of circ-RBM33 in human nasopharyngeal carcinoma CNE1 cells was higher than that in human nasopharyngeal mucosal epithelial HNEpC cells,while the relative expression of miR-383-3p was lower than that in HNEpC cells(P<0.05).In the si-RBM33 group,the relative expression of circ-RBM33 in CNE1 cells,cell viability,number of invading cells,scratch closure rate,and expression of CKS1B protein in cells were lower than those in the si-NC group(P<0.05).In the si-RBM33+anti-miR-383-3p group,the cell viability,number of invading cells,scratch closure rate,and expression of CKS1B protein in cells were higher than those in the si-RBM33 group(P<0.05).In the miR-383-3p group,the relative expres-sion of miR-383-3p in CNE1 cells was significantly increased,while cell viability,number of invading cells,scratch closure rate,and expression of CKS1B protein in cells were lower than those in the miR-NC group(P<0.05).Transfection with WT-circ-RBM33/CKS1B resulted in significantly lower luciferase activity in the miR-383-3p group than in the miR-NC group(P<0.0001).Conclusion:Knockdown of circ-RBM33 may inhibit the proliferation,invasion,and migration of nasopharyngeal carcinoma cells by upregulating miR-383-3p expression and subsequently suppressing CKS1B expression.
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