Effect of Phloretin on the Malignant Progression of Lung Cancer Cells by Regulating the CCL2-CCR2 Signaling Axis
Objective:To investigate the effect of phloretin on the CC chemokine ligand 2(CCL2)-CC chemokine receptor 2(CCR2)signaling axis on the malignant progression of lung cancer cells.Methods:A549 cells were grouped into a control group(untreated A549 cells),a low-dose phloretin group(500 µmoL/L phloretin treated A549 cells),a medium-dose phloretin group(750µmoL/L phloretin treated A549 cells),a high-dose phloretin group(1000µmoL/L phloretin treated A549 cells),and a high-dose phloretin+GW0742 group(1000µmoL/L phloretin and 1.0μmoL/L CCL2-CCR2 signaling pathway activator GW0742 co-treated A549 cells).CCK-8 method and flow cytometry were applied to detect A549 cell proliferation and apoptosis,respectively;the Transwell method was applied to detect cell migration and invasion;Western blot was applied to determine the expression of CCL2-CCR2 signaling axis-related proteins.Results:The OD450 value,migration and invasion numbers,and the CCL2,CCR2 protein expression in the low,medium,and high dose phloretin groups were lower than those in the control group(P＜0.05),the apoptosis rate of cells was higher(P＜0.05),in a dose-dependent manner.The OD450 value,migration and invasion numbers,and the CCL2,CCR2 protein expression in the high dose phloretin+GW0742 group were higher than those in the control group(P＜0.05),the apoptosis rate of cells was lower than that in the control group(P＜0.05).Conclusion:Phloretin may inhibit the malignant progression of lung cancer cells by regulating the CCL2-CCR2 signaling axis-mediated immune response.
万方数据
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