Combined Detection of High-Mobility Group Box 1 Protein Macrophage Migration Inhibitory Factor(MIF)and CXC Chemokine Subfamily 16 for the Diagnosis of High-Risk Human Papillomavirus Infection
Objective:To investigate the expression levels and diagnostic value of high-mobility group box 1 protein(HMGB1),macrophage migration inhibitory factor(MIF),and CXC chemokine subfamily 16(CXCL16)in patients with high-risk human papillomavirus(HPV)infection.Methods:A total of 150 pa-tients with HPV infection admitted to our hospital from October 2020 to October 2023 were enrolled in the study group and divided into high-risk HPV infection group(n=40)and low-risk HPV infection group(n=110)according to the results of nucleic acid detection.Another 100 healthy women who underwent transvagi-nal colposcopy in our hospital during the same period were selected as the control group.The HPV-DNA gen-otyping kit was used to detect HPV in the study group patients;enzyme-linked immunosorbent assay(ELISA)was used to detect the levels of HMGB1 and CXCL16 in the study group and control group;and im-munohistochemistry was used to detect the staining intensity of MIF.The differences in HMGB1,MIF,and CXCL16 between the control group and the study group were compared;the differences in HMGB1,MIF,and CXCL16 between the low-risk HPV infection group and the high-risk HPV infection group were compared;receiver operating characteristic(ROC)curve analysis was used to evaluate the diagnostic value of HMGB 1,MIF,and CXCL16 combined detection for high-risk HPV infection;Spearman correlation analysis was used to analyze the correlation between HMGB1,MIF,and CXCL16 and different HPV infection genotypes.Results:Compared with the control group,the levels of serum HMGB1 and CXCL16,and the score of MIF positive cell proportion in the study group were significantly higher(all P＜0.05);compared with the low-risk HPV infec-tion group,the levels of serum HMGB1 and CXCL16,and the score of MIF positive cell proportion in the high-risk HPV infection group were significantly higher(all P＜0.05);ROC curve analysis showed that the AUC of HMGB1,MIF,and CXCL16 for the diagnosis of high-risk HPV infection alone and in combination were 0.748,0.684,0.791,and 0.934,respectively.The diagnostic value of combined detection was significantly higher than that of single detection(Z=2.577,3.152,2.096,all P＜0.05);Spearman correlation analysis showed that HMGB1,MIF,and CXCL16 were significantly positively correlated with different HPV infection genotypes(r=0.615,0.633,0.649,all P＜0.05).Conclusion:The levels of serum HMGB1 and CXCL16,and the score of MIF positive cell proportion in patients with high-risk HPV infection were significantly higher than those in the low-risk and control groups.The combined detection of HMGB1,MIF,and CXCL16 in clin-ical examination can improve the clinical diagnosis rate of high-risk HPV infection and provide a new detec-tion method and treatment basis for clinical practice.
Human papillomavirusGenotypingInfectionHigh-mobility group box 1 pro-teinMacrophage migration inhibitory factorCXC chemokine subfamily 16
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