Role of the NKILA-miR-889-3p Axis in Regulating Proliferation Migration and Invasion of Oral Squamous Cell Carcinoma Cells
Objective:To analyze the expression and clinical significance of the long non-coding RNA NKILA in oral squamous cell carcinoma(OSCC),investigate the regulatory mechanism between NKILA and its potential target miRNA,and elucidate its role in OSCC development.Methods:Eighty OSCC patients con-firmed by pathology and corresponding adjacent normal oral mucosa tissue samples were collected.The expres-sion level of NKILA and its correlation with prognosis were detected using qRT-PCR.The effects of NKILA on OSCC cell proliferation,migration,and invasion were evaluated through colony formation assays and Transwell assays.Subcellular fractionation experiments were conducted to determine the subcellular localization of NKI-LA.LncBase was used to predict potential target miRNAs of NKILA.AGO2 immunoprecipitation experiments were performed to validate the interaction between miR-889-3p and NKILA.Results:NKILA expression was significantly downregulated in OSCC tissues compared to adjacent normal tissues(P＜0.0001).Low expres-sion of NKILA was significantly associated with advanced TNM stage,lymph node metastasis,and distant me-tastasis in OSCC patients(P=0.001,P=0.046,P=0.011).Cell experiments demonstrated that knockdown of NKILA significantly promoted OSCC cell migration and invasion(P＜0.05),while overexpression of NKILA significantly inhibited these cellular functions(P＜0.05).Subcellular localization experiments showed that NKILA primarily localized in the cytoplasm.miR-889-3p was identified as a potential target of NKILA,and overexpression of NKILA significantly upregulated miR-889-3p expression(P＜0.05),and vice versa.AGO2 immunoprecipitation experiments further confirmed that miR-889-3p specifically binds to and regulates NKILA(P＜0.05).Conclusion:This study elucidates the downregulation of NKILA in OSCC development and its potential functional mechanisms.It reveals an important regulatory interaction between NKILA and miR-889-3p,providing new insights for the study and treatment of OSCC.
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