Effect of Paeoniflorin on Hydrogen Peroxide-Induced Oxidative Stress Damage in Skin Fibroblasts by Regulating the SIRT1/PGC-1α/Nrf2 Signaling Pathway
Objective:To investigate the effect of paeoniflorin(PF)on oxidative stress damage induced by hydrogen peroxide(H2O2)in skin fibroblasts(HSF)by regulating the silent information regulator 2 homo-logue 1(SIRT1)/peroxisome proliferator activated receptor-gamma coactivator-1α(PGC-1α)/nuclear fac-tor erythroid-derived 2-related factor 2(Nrf2)signaling pathway.Methods:HSF cells were studied and sep-arated into the Control group,H2O2 group,L-PF,M-PF,H-PF group,and PF+EX527 group.CCK-8 was applied to detect HSF cell proliferation.The β-galactosidase staining method was applied to detect HSF cell aging.Flow cytometry was used to detect apoptosis of HSF cells.ELISA method was used to detect the expres-sion of ROS,SOD,GSH,and MDA in HSF cells.WB was used to detect the expressions of SIRT1,PGC-1α,and Nrf2 proteins in HSF cells.Results:The survival rate,SOD,GSH,SIRT1,PGC-1α,and Nrf2 ex-pression of HSF cells in the H2O2 group were lower than in the control group,the proportion of aging cells,apoptosis rate,ROS,and MDA expression were higher than in the control group(P<0.05).Compared with the H2O2 group,the survival rate,SOD,GSH,SIRT1,PGC-1α,and Nrf2 expression were increased in the L-PF group,M-PF group,and H-PF group,the proportion of aging cells,apoptosis rate,ROS,and MDA expression were decreased(P<0.05).The survival rate,SOD,GSH,SIRT1,PGC-1α,and Nrf2 expression in the PF+EX527 group were lower than in the H-PF group,the proportion of aging cells,apoptosis rate,ROS,and MDA expression were higher than in the H-PF group(P<0.05).Conclusion:PF can inhibit H2O2-induced oxidative stress damage in HSF cells,and its mechanism may be achieved by activating the SIRT1/PGC-1α/Nrf2 signaling pathway.
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