摘要
目的:探讨芍药苷(PF)调节沉默信息调节因子 2 同系物 1(SIRT1)/氧化物酶体增殖物激活受体γ共激活剂-1α(PGC-1α)/核因子E2 相关因子2(Nrf2)信号通路对过氧化氢(H2O2)诱导皮肤成纤维细胞(HSF)氧化应激损伤的影响.方法:以 HSF 细胞为研究对象,将其分为 Control 组、H2O2组、L-PF、M-PF、H-PF组、PF+EX527 组;CCK-8 检测 HSF 细胞增殖;β-半乳糖苷酶染色法检测 HSF细胞衰老;流式细胞仪检测HSF细胞凋亡;ELISA法检测HSF细胞中ROS、SOD、GSH、MDA的表达;WB检测HSF细胞中 SIRT1、PGC-1α、Nrf2 蛋白表达.结果:H2O2 组 HSF 细胞的存活率、SOD、GSH、SIRT1、PGC-1α、Nrf2 表达低于Control组,衰老细胞比例、凋亡率、ROS、MDA表达高于Control组(P<0.05);与H2O2 组比较,L-PF 组、M-PF 组、H-PF 组存活率、SOD、GSH、SIRT1、PGC-1α、Nrf2 表达升高,衰老细胞比例、凋亡率、ROS、MDA 表达降低(P<0.05);PF+EX527 组存活率、SOD、GSH、SIRT1、PGC-1α、Nrf2 表达低于H-PF组,衰老细胞比例、凋亡率、ROS、MDA表达高于H-PF组(P<0.05).结论:PF可以抑制H2O2 诱导的HSF细胞氧化应激损伤,其机制可能是激活SIRT1/PGC-1α/Nrf2 信号通路实现的.
Abstract
Objective:To investigate the effect of paeoniflorin(PF)on oxidative stress damage induced by hydrogen peroxide(H2O2)in skin fibroblasts(HSF)by regulating the silent information regulator 2 homo-logue 1(SIRT1)/peroxisome proliferator activated receptor-gamma coactivator-1α(PGC-1α)/nuclear fac-tor erythroid-derived 2-related factor 2(Nrf2)signaling pathway.Methods:HSF cells were studied and sep-arated into the Control group,H2O2 group,L-PF,M-PF,H-PF group,and PF+EX527 group.CCK-8 was applied to detect HSF cell proliferation.The β-galactosidase staining method was applied to detect HSF cell aging.Flow cytometry was used to detect apoptosis of HSF cells.ELISA method was used to detect the expres-sion of ROS,SOD,GSH,and MDA in HSF cells.WB was used to detect the expressions of SIRT1,PGC-1α,and Nrf2 proteins in HSF cells.Results:The survival rate,SOD,GSH,SIRT1,PGC-1α,and Nrf2 ex-pression of HSF cells in the H2O2 group were lower than in the control group,the proportion of aging cells,apoptosis rate,ROS,and MDA expression were higher than in the control group(P<0.05).Compared with the H2O2 group,the survival rate,SOD,GSH,SIRT1,PGC-1α,and Nrf2 expression were increased in the L-PF group,M-PF group,and H-PF group,the proportion of aging cells,apoptosis rate,ROS,and MDA expression were decreased(P<0.05).The survival rate,SOD,GSH,SIRT1,PGC-1α,and Nrf2 expression in the PF+EX527 group were lower than in the H-PF group,the proportion of aging cells,apoptosis rate,ROS,and MDA expression were higher than in the H-PF group(P<0.05).Conclusion:PF can inhibit H2O2-induced oxidative stress damage in HSF cells,and its mechanism may be achieved by activating the SIRT1/PGC-1α/Nrf2 signaling pathway.
基金项目
湖北省武汉市医学科研项目立项任务书(WX20Q03)
国家科技期刊平台
NSTL
万方数据