Plantamajoside Attenuates LPS-Induced Inflammatory Response of Human Gingival Fibroblasts by Inhibiting the NF-κB/IL-6 Signaling Pathway
Objective:To explore the effect of plantamajoside(PMS)on the inflammatory response of human gingival fibroblasts induced by lipopolysaccharide(LPS)by inhibiting nuclear factor-κB(NF-κB)/interleukin-6(IL-6)signaling pathway.Methods:The cultured human gingival fibroblasts(HGnF)were randomly divided into control group,model group(10mg/L LPS),PMS low and high-dose groups(50,100μg/mL PMS+10mg/L LPS),activator group(10μM BAY11-7085+10mg/L LPS),and PMS high-dose+activator group(10μM BAY11-7085+100μg/mL+10mg/L LPS).CCK-8 method was used to detect HG-nF cell viability,ELISA method was used to detect the contents of interleukin 1β(IL-1β),interleukin 6(IL-6),and interleukin 18(IL-18)in the supernatant of HGnF cells.Western blot method was used to detect the expression levels of inducible nitric oxide synthase(iNOS),IL-1β and NF-κB/IL-6 signaling pathway-related proteins.Results:Low and high-doses PMS could increase the cell viability of HGnF induced by LPS,decrease the contents of IL-1β,IL-6,IL-18 in supernatant,and the expression levels of IL-1β,iNOS,p-NF-κB/NF-κB,and IL-6 in cells(P<0.05).The activator BAY11-7085 reversed the effects of high-dose PMS on the above indicators in LPS-induced HGnF cells(P<0.05).Conclusion:PMS may improve the in-flammation of HGnF cells induced by LPS by inhibiting the NF-κB/IL-6 signaling pathway.
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